Cleaved-Caspase-1 p20 (D210) Polyclonal Antibody

YP-Ab-00001

种属

Human;Mouse;Rat

应用

WB;IF;IHC;ELISA

基因名称（Gene Name）

       CASP1

蛋白名称

Caspase1

人基因ID

834

人蛋白质序列数据库

       P29466

小鼠蛋白质序列数据库

       P29452

免疫原

The antiserum was produced against synthesized peptide derived from human Caspase-1. AA range:161-210

特异性

Cleaved-Caspase-1 (D210) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 protein resulting from cleavage adjacent to D210.

组成（Formulation）

       Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源

      Polyclonal, Rabbit,IgG

稀释比例

WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300

纯化工艺（Immunogen）

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

浓度

       1 mg/ml

储存（Storage）

       -20°C/1 year

其他名称

       CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45

分子量（DA）

25kD

背景（Background）

 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012],

功能

       alternative products:Additional isoforms seem to exist,catalytic activity:Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.,enzyme regulation:Specifically inhibited by the cowpox virus Crma protein.,function:Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.,PTM:The two subunits are derived from the precursor sequence by an autocatalytic mechanism.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subu

细胞定位

Cytoplasm . Cell membrane .

组织表达

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.